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体外 细胞毒性淋巴细胞杀伤疟 原虫感染红细胞的测定
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
In Vitro Assay of Plasmodium-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes
DOI:

08:20 min

August 17, 2022

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Capitoli

  • 00:04Introduction
  • 00:58Experimental Malaria Infection in Mouse
  • 01:49Obtaining Fresh Whole Mouse Splenocytes
  • 03:30Cytotoxic Effector Cell Purification: CD8a+ T Cell Negative Selection
  • 04:22P. yoelii Infected RBC Isolation
  • 05:21CFSE Labeling of RBCs, Preparation for Flow Cytometry and Cytotoxic Lymphocyte Coculture
  • 06:22Results: P. yoelii – iRBC Enrichment and Assessment of cell lysis Percentage of P. yoelii – iRBCs by CD8+ lymphocytes
  • 07:39Conclusion

Summary

Traduzione automatica

在这里,我们描述了一种新方法,以帮助阐明感染血液阶段细胞对 疟原虫 免疫的机制。这是一种 体外 测定,用于测量细胞毒性淋巴细胞对感染红细胞的杀伤。

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