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Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates
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Capitoli
- 00:05Introduction
- 00:49Fabrication of 3D Cell Culture Substrates
- 02:26Substrate Passivation of 3D Cell Culture Substrates
- 04:01UV-Photopatterning of 3D Cell Culture Substrates
- 05:27Protein Solution Preparation and Incubation
- 06:18Cell Seeding and Image Acquisition
- 07:16Results: Analysis of Human Dermal Fibroblasts and Human Primary Keratocytes on Patterned Surfaces
- 08:51Conclusion
Traditionally, cell culture is performed on planar substrates that poorly mimic the natural environment of cells in vivo. Here we describe a method to produce cell culture substrates with physiologically relevant curved geometries and micropatterned extracellular proteins, allowing systematic investigations into cellular sensing of these extracellular cues.
