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Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues
JoVE Journal
Biologia
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JoVE Journal Biologia
Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues
DOI:

09:57 min

October 17, 2022

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Capitoli

  • 00:05Introduction
  • 01:20Preparation of the Plasma and the Maxillary Bone Samples and Extraction of Extracellular Vesicles
  • 02:50Origin Analysis of Extracellular Vesicles
  • 05:17Protein Content Analysis of Extracellular Vesicles
  • 08:06Results: Profiling of Surface Antigens and Protein Cargos of Extracellular Vesicles Isolated from In Vivo Samples
  • 09:13Conclusion

Summary

Traduzione automatica

The present protocol describes a method to extract extracellular vesicles from the peripheral blood and solid tissues with subsequent profiling of surface antigens and protein cargos.

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