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Test dla Przyczepność i Invasion Agar w S. cerevisiae
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JoVE Journal Biologia
Assay for Adhesion and Agar Invasion in S. cerevisiae

Test dla Przyczepność i Invasion Agar w S. cerevisiae

DOI:
10.3791/64-v

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04:36 min

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November 08, 2006

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,
1Department of Molecular Biology,Princeton University

Capitoli

  • 00:04Preparation of plates
  • 01:01Adhesion assay
  • 02:40Invasion assay
  • 04:04Phenotype observations under microscope

Summary

Traduzione automatica

Traduzione automatica

Opisujemy to test na przyczepność drożdży i inwazji agar jako miara zróżnicowania inwazyjne i pseudohyphal. Ten prosty test może być wykorzystane do oceny inwazyjnym fenotypem różnych mutantów, jak również wpływu na środowisko pamięci i szlaków sygnałowych różnicowania drożdży.

Tags

AdhesionAgar InvasionS. CerevisiaeBiofilmsIndustrial ProductivityStructuresHuman LifeSustainable EnergyColonizationSignaling PathwaysEnvironmental CuesModel OrganismRas-PKA PathwayKss1 MAPK PathwayHog1 Osmolarity PathwayPseudohyphal FilamentsSignal Transduction ResearchFilamentationPhenotypesMAT Formation AssayColony SpreadSoft AgarCrystal Violet St

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