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En simpel pitanalyseprotokol til visualisering og kvantificering af osteoklastisk resorption in vitro
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Medicina
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JoVE Journal Medicina
A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

En simpel pitanalyseprotokol til visualisering og kvantificering af osteoklastisk resorption in vitro

DOI:
10.3791/64016-v

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07:03 min

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June 16, 2022

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, , , ,
1Department of Oral and Maxillofacial Surgery,University Hospital Tübingen, 2Department of Thoracic and Cardiovascular Surgery,University Hospital Tübingen, 3Department of Orthopaedic Surgery,University Hospital Tübingen

Capitoli

  • 00:04Introduction
  • 00:51Calcification of 96-Well Cell Culture Plates
  • 01:54Isolation of Peripheral Blood Mononuclear Cells (PBMCs)
  • 02:49Expansion of Osteoclasts Progenitors and Induction of Osteoclastogenesis
  • 04:19Fluorescence Staining of Osteoclasts and Calcium Phosphate Coating
  • 05:17Results: Analysis of Mature Osteoclast Cultured on Calcium Phosphate Coated Wells
  • 06:38Conclusion

Summary

Traduzione automatica

Traduzione automatica

Her præsenterer vi en enkel og effektiv analyseprocedure for resorptionsgrubeassays ved hjælp af calciumphosphatbelagte cellekulturplader.

Tags

Osteoclastic ResorptionCalcium Phosphate CoatingPBMCOsteoclast DifferentiationBone Marrow Derived MonocytesRaw 264.7 CellsDensity Gradient CentrifugationMacrophage Colony Stimulating Factor

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