/

/

体外重组巨型单层囊泡内的肌动蛋白细胞骨架

JoVE Journal
Bioingegneria

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Bioingegneria

In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles

Please note that all translations are automatically generated. Click here for the English version.

体外重组巨型单层囊泡内的肌动蛋白细胞骨架

DOI:

10.3791/64026-v

•

10:19 min

•

August 25, 2022

•

Sheng Chen, Zachary Gao Sun³, Michael P. Murrell^2,3

¹Department of Biomedical Engineering,Yale University, ²Systems Biology Institute,Yale University, ³Department of Physics,Yale University

Capitoli

00:04Introduction
00:50Preparation of Buffers and Proteins Solutions
03:07Preparation of Liposomes Based on the Inverted Emulsion Techniques
05:51Microscopy Observation
08:11Results: Microscopic Images of the Monolayer Lipid Droplet and Encapsulation of Actin Networks Inside Liposomes
09:27Conclusion

Summary

Traduzione automatica

在这份手稿中，我们展示了将F-肌动蛋白细胞骨架封装到巨大的单层脂质囊泡（也称为脂质体）中的实验技术，以及在脂质体膜的内小叶处形成皮质仿生F-肌动蛋白层的方法。

Tags

Actin Cytoskeleton In Vitro Reconstitution Giant Unilamellar Vesicles Liposome Yield Encapsulation Efficiency Protein Encapsulation Microparticle Encapsulation Self-propelling Microswimmers Non-polymerization Buffer Polymerization Buffer EGGPC Nickel Lipid

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

眼刺激试验（EIT）使用重构型人角膜上皮样（RHCE）组织模型眼刺激性化学品危险性鉴定

一个<em>体外</em鼠类的椎间盘>器官培养模型

倒置方向下整个人的心脏在加压袋内的去细胞化

长期纳米材料暴露后体外基因毒性测试的高级 3D 肝脏模型

测量组织直肠炎和莱蒂克药物血栓分析功效的体外系统

标记细胞外囊泡以监测软骨外植体的迁移和摄取

巨型单层囊泡内重组细胞骨架的快速封装

用市售蛋白质重建基于肌动蛋白的运动

（poro-）弹性收缩肌动球蛋白网络作为细胞骨架模型系统的机制

Dynamic Light-Induced Protein Patterns at Model Membranes

Read Article