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Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
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Biologia
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JoVE Journal Biologia
Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

DOI:
10.3791/64028-v

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09:10 min

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July 27, 2022

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, , , ,
1Department of Medicine,Renaissance School of Medicine at Stony Brook University, 2Department of Physiology and Biophysics,Renaissance School of Medicine at Stony Brook University

Capitoli

  • 00:04Introduction
  • 01:24Preparation
  • 02:41Total‐Body Gamma Irradiation and Tissue Collection
  • 06:22Results: Analysis of Small Intestine Sections after Irradiation
  • 08:18Conclusion

Summary

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The gastrointestinal tract is one of the most sensitive organs to injury upon radiotherapeutic cancer treatments. It is simultaneously an organ system with one of the highest regenerative capacities following such insults. The presented protocol describes an efficient method to study the regenerative capacity of the intestinal epithelium.

Tags

Intestinal Epithelial RegenerationIonizing RadiationGamma IrradiationColorectal CancerLineage TracingTamoxifenEdUBouin’s FixativeGamma IrradiatorCre-mediated Recombination

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