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一种半自动且可重现的基于生物学的体外定量钙沉积的方法
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JoVE Journal Biochimica
A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
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一种半自动且可重现的基于生物学的体外定量钙沉积的方法

DOI:
10.3791/64029-v

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11:30 min

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June 02, 2022

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, , , , , ,
1Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM),Maastricht University, 2Helmholtz Institute for Biomedical Engineering, Biointerface Group,RWTH Aachen University, 3Institute of Experimental Medicine and Systems Biology,RWTH Aachen University

Capitoli

  • 00:05Introduction
  • 00:36Cell Seeding, Maintenance, and Calcification Induction
  • 02:13Calcification Detection via Imaging
  • 07:51Data Analysis
  • 10:13Results: A Semi-Automated Method for In Vitro Calcification Determination
  • 11:05Conclusion

Summary

Traduzione automatica

Traduzione automatica

心血管疾病是全世界死亡的主要原因。血管钙化大大加重了心血管发病率和死亡率的负担。该协议描述了一种通过荧光成像在 体外 量化血管平滑肌细胞介导的钙沉淀的简单方法。

Tags

CalcificationVascular Smooth Muscle CellsFluorescent-based DetectionHVSMCSCalcification MediumDAPIRFPBright FieldImagingData Reduction

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