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Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells
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Capitoli
- 00:10Introduction
- 00:50Making the Macroporous Alginate Scaffolds
- 05:47Transduction
- 10:07Risultati
- 10:51Conclusions
Herein is a protocol for creating dry macroporous alginate scaffolds that mediate efficient viral gene transfer for use in genetic engineering of T cells, including T cells for CAR-T cell therapy. The scaffolds were shown to transduce activated primary T cells with >85% transduction.
