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干式大孔海藻酸盐支架的制备和使用用于T细胞的病毒转导

JoVE Journal
Bioingegneria
Author Produced

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JoVE Journal Bioingegneria

Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells

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干式大孔海藻酸盐支架的制备和使用用于T细胞的病毒转导

DOI:

10.3791/64036-v

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11:16 min

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September 09, 2022

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Madelyn VanBlunk, Pritha Agarwalla², Sharda Pandit², Yevgeny Brudno^2,3

¹Joint Department of Biomedical Engineering,University of North Carolina at Chapel Hill and North Carolina State University, ²Comparative Medicine Institute,North Carolina State University, ³Lineberger Comprehensive Cancer Center,University of North Carolina at Chapel Hill

Capitoli

00:10Introduction
00:50Making the Macroporous Alginate Scaffolds
05:47Transduction
10:07Risultati
10:51Conclusions

Summary

Traduzione automatica

本文是用于创建干燥的大孔藻酸盐支架的方案，该支架介导有效的病毒基因转移，用于T细胞的基因工程，包括用于CAR-T细胞治疗的T细胞。支架被证明以>85%的转导转导活化的原代T细胞。

Tags

Dry Macroporous Alginate Scaffolds Drydux Viral Transduction T Cells Spinoculation RetroNectin Calcium-D-gluconate Alginate Solution Lyophilization 48-well Plate 24-well Plate

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