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研究鞘氨醇1-磷酸受体结构和信号通路的管道

JoVE Journal
Biochimica

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JoVE Journal Biochimica

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

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研究鞘氨醇1-磷酸受体结构和信号通路的管道

DOI:

10.3791/64054-v

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12:27 min

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June 08, 2022

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Lin Cheng*¹, Lantian Su*¹, Xiaowen Tian*¹, Fan Xia, Chang Zhao, Wei Yan, Zhenhua Shao

¹Division of Nephrology and Kidney Research Institute, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital,Sichuan University, ²Department of Neurosurgery, West China Hospital,Sichuan University

Capitoli

00:05Introduction
01:28Purification of Sphingosine 1-Phosphate Receptors (S1PRs)-G Protein Complex
02:16Electron Microscopy to Resolve the S1PRs Structure
07:56S1PRs-Gi Mediated cAMP Inhibition Assay
10:33Results: Purification and Analysis of S1PRs-Gi Complex
11:48Conclusion

Summary

Traduzione automatica

S1P通过S1P受体（S1PRs）亚家族发挥其多样化的生理作用。这里描述了一个管道来阐述S1PR的结构和功能。

Tags

Sphingosine 1-phosphate Receptors S1PR Activation Drug Development Cryo-electron Microscopy Protein Purification Size Exclusion Chromatography 2D Classification Image Processing Auto-picking

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