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Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
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Capitoli
- 00:04Introduction
- 00:47Centrifugation of the Oocytes
- 01:51Preparation of the Oocyte for Bisection
- 03:00Bisection of the Oocytes
- 04:56Results: Successful and Unsuccessful qPCR Results and Boxplot from Mitochondria-Reduced and Mitochondria-Dense Ooplasts
- 05:39Conclusion
Here, we present a protocol to significantly reduce the mitochondrial DNA copy numbers in a bovine oocyte (P < 0.0001). This method utilizes centrifugation and bisection to substantially reduce oocyte mitochondria and may allow for an increased chance of development in the reconstructed interspecies somatic cell nuclear transfer embryos.
