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使用离体灌注生物反应器进行大鼠后肢的获取和去细胞化血管化复合同种异体移植

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Bioingegneria

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JoVE Journal Bioingegneria

Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation

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使用离体灌注生物反应器进行大鼠后肢的获取和去细胞化血管化复合同种异体移植

DOI:

10.3791/64069-v

•

08:50 min

•

June 09, 2022

•

Aisha Adil², Golnaz Karoubi³, Siba Haykal^2,4

¹Latner Thoracic Research Surgical Laboratories,University Health Network, Toronto General Hospital, ²Institute of Medical Science, Temerty Faculty of Medicine,University of Toronto, ³Institute of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine,University of Toronto, ⁴Division of Plastic & Reconstructive Surgery, Department of Surgery,University of Toronto

Capitoli

00:05Introduction
00:38Procurement of Rat Hindlimb
02:21Decellularization of Rat Hindlimbs
05:49Post-Decellularization Washing and Sterilization
06:55Results: Histological Staining and DNA Quantification of Native and Decellularized Rat Hindlimb Tissues
08:13Conclusion

Summary

Traduzione automatica

我们描述了复合大鼠后肢的手术技术和去细胞化过程。使用低浓度十二烷基硫酸钠通过离体机灌注系统进行脱细胞。

Tags

Keywords: Procurement Decellularization Rat Hindlimbs Ex Vivo Perfusion-based Bioreactor Vascularized Composite Allotransplantation Immunosuppression Femoral Vessels Cannulation Heparinized Saline Bioreactor Peristaltic Tubing

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