Rivista
/
Biologia
/
Viral transgenekspression i gnaverhjerter og vurdering af hjertearytmirisiko
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Viral Transgene Expression in Rodent Hearts and the Assessment of Cardiac Arrhythmia Risk

Viral transgenekspression i gnaverhjerter og vurdering af hjertearytmirisiko

DOI:
10.3791/64073-v

•

05:15 min

•

July 27, 2022

•

, , , , , , , ,
1University of Ottawa Heart Institute, 2Department of Cellular and Molecular Medicine,University of Ottawa, 3Department of Biochemistry, Microbiology and Immunology,University of Ottawa

Capitoli

  • 00:05Introduction
  • 00:51Viral Transgene Expression in Rodent Ventricular Tissues
  • 01:37Assessment of Cardiac Arrhythmia Susceptibility
  • 03:47Results: Assessment of Cardiac Susceptibility to Arrhythmias
  • 04:35Conclusion

Summary

Traduzione automatica

Traduzione automatica

Denne protokol beskriver metoder til transgenekspression i rotte- og musehjerter ved direkte intramyokardieinjektion af virus under ekkokardiografisk vejledning. Metoder til vurdering af hjerters modtagelighed for ventrikulære arytmier ved programmeret elektrisk stimulering af isolerede, Langendorff-perfunderede hjerter forklares også her.

Tags

Keywords: Viral Transgene ExpressionVentricular MyocardiumCardiac ArrhythmiaLangendorff PerfusionECGIsoproterenolProgrammed Electrical StimulationVentricular Tachyarrhythmia

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/64073?article_type=v

Targeted Expression of GFP in the Hair Follicle Using Ex Vivo Viral Transduction
check_url/it/64073?article_type=v

Optical Mapping of Langendorff-perfused Rat Hearts
check_url/it/64073?article_type=v

Semi-automated Optical Heartbeat Analysis of Small Hearts
check_url/it/64073?article_type=v

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes
check_url/it/64073?article_type=v

Respirometric Oxidative Phosphorylation Assessment in Saponin-permeabilized Cardiac Fibers
check_url/it/64073?article_type=v

In vitro Reconstitution of the Active T. castaneum Telomerase
check_url/it/64073?article_type=v

Vurdering af GFP Expression og levedygtighed Brug af Tali Billed-Based cytometer
check_url/it/64073?article_type=v

Intravital mikroskopi af mikrocirkulationen i Mouse Cremaster Muscle for Analyse af perifert Stem Cell Migration
check_url/it/64073?article_type=v

In vitro-syntese af modificeret mRNA for Induktion af protein-ekspression i humane celler
check_url/it/64073?article_type=v

En protokol til brug af gen sæt berigelse analyse til at identificere den passende dyremodel for Translationel forskning

Read Article