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Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans
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Biologia
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JoVE Journal Biologia
Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans

Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans

DOI:
10.3791/64092-v

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07:25 min

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December 09, 2022

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, , ,
1Graduate Program in Developmental Biology,Baylor College of Medicine, 2Huffington Center on Aging,Baylor College of Medicine, 3Integrative Program of Molecular and Biochemical Sciences,Baylor College of Medicine, 4Department of Molecular and Human Genetics,Baylor College of Medicine, 5Howard Hughes Medical Institute,Baylor College of Medicine

Capitoli

  • 00:04Introduction
  • 00:45Preparation of Lipid‐Conditioned Bacteria
  • 02:00Lipid Supplementation for Caenorhabditis elegans
  • 03:37RNA Extraction from a Small Number of Whole Worms
  • 05:53Results: Validation of Transcriptional Changes, Transcriptional Evaluations, and Lifespan Assay Upon Lipid Supplementation
  • 06:41Conclusion

Summary

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The present protocol describes lipid supplementation methods in liquid and on-plate cultures for Caenorhabditis elegans, coupled with longitudinal studies and gene transcriptional analysis from bulk or a few worms and worm tissues.

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Keywords: Lipid SupplementationLongevityGene Transcriptional AnalysisCaenorhabditis ElegansOP50 BacteriaBacterial DilutionDietary RestrictionBDR MediumLipid Stock SolutionLiquid CultureSynchronized Caenorrhabditis ElegansNGM Agar PlateTranscriptional ChangesLiquid FeedingHydro Techniques

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