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Live Imaging of Microtubule Dynamics in Glioblastoma Cells Invading the Zebrafish Brain
 

Live Imaging of Microtubule Dynamics in Glioblastoma Cells Invading the Zebrafish Brain

Article DOI: 10.3791/64093-v • 09:29 min • July 29th, 2022
July 29th, 2022 •
1, 1, 1
1Cell Polarity, Migration and Cancer Unit, Université Paris-Cité, Institut Pasteur, Équipe Labellisée Ligue Contre le Cancer

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We report a technique permitting live imaging of microtubule dynamics in glioblastoma (GBM) cells invading a vertebrate brain tissue. Coupling the orthotopic injection of fluorescently tagged GBM cells into a transparent zebrafish brain with high-resolution intravital imaging allows the measurement of cytoskeleton dynamics during in situ cancer invasion.

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Keywords: Microtubule Dynamics Glioblastoma Cells Zebrafish Brain Subcellular Intravital Imaging Brain Cancer Invasion Protein Analysis Cell Transplantation Microinjection Anesthetized Larvae
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