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JoVE Journal Neuroscience
Helhjerne enkeltcellebilleddannelse og analyse af intakte neonatale musehjerner ved hjælp af MR, vævsrensning og lysarkmikroskopi
 
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Helhjerne enkeltcellebilleddannelse og analyse af intakte neonatale musehjerner ved hjælp af MR, vævsrensning og lysarkmikroskopi

Article DOI: 10.3791/64096-v • 08:49 min • August 1st, 2022
August 1st, 2022 •
*1,2, *1,2, 1,2, 1,2, 3, 3,7, 7, 4,5,6, 4,5,6, 3, 4,5,6, 1,2
1UNC Neuroscience Center, University of North Carolina, Chapel Hill, 2Department of Genetics, University of North Carolina, Chapel Hill, 3Department of Psychiatry, University of North Carolina, Chapel Hill, 4Center for Animal Magnetic Resonance Imaging, The University of North Carolina at Chapel Hill, 5Biomedical Research Imaging Center, The University of North Carolina at Chapel Hill, 6Department of Neurology, The University of North Carolina at Chapel Hill, 7Department of Computer Science, The University of North Carolina at Greensboro
* These authors contributed equally

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Denne protokol beskriver metoder til udførelse af magnetisk resonansbilleddannelse, rydning og immunmærkning af intakte musehjerner ved hjælp af iDISCO+, efterfulgt af en detaljeret beskrivelse af billeddannelse ved hjælp af lysarkmikroskopi og nedstrømsanalyser ved hjælp af NuMorph.

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Neurovidenskab udgave 186
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