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清醒小鼠小胶质细胞动力学和神经元活动的同时成像
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal Neuroscienze
Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
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清醒小鼠小胶质细胞动力学和神经元活动的同时成像

DOI:
10.3791/64111-v

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08:26 min

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August 23, 2022

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, , , ,
1Department of Cellular Neurobiology, Graduate School of Medicine and Faculty of Medicine,The University of Tokyo

Capitoli

  • 00:04Introduction
  • 01:00AAV Injection
  • 03:04Cranial Window Implantation
  • 04:36In Vivo Two‐Photon Imaging of Microglia and Neuronal Calcium Dynamics
  • 06:42Results: Microglial Dynamics and Visual Responses in a Dendritic Spine
  • 07:44Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们描述了一种将腺相关病毒注射与颅窗植入相结合的方案，用于同时对清醒小鼠的小胶质细胞动力学和神经元活动进行成像。

Tags

Simultaneous ImagingMicroglia DynamicsNeuronal ActivityAwake MiceAAV InjectionCranial WindowStereotaxic InstrumentMotion ArtifactPrimary Visual CortexMicroinjector

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