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用于下游无循环DNA应用的标准化液体活检前分析方案

JoVE Journal
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JoVE Journal Ricerca sul cancro

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

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用于下游无循环DNA应用的标准化液体活检前分析方案

DOI:

10.3791/64123-v

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05:26 min

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September 16, 2022

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Julie Earl, Silvia Calabuig-Fariñas, María Eugenia Sarasquete, Laura Muinelo Romay, Sara Lopez-Tarruella, Beatriz Bellosillo Paricio, Marta Rodríguez, Karmele Valencia Leoz, Marta Dueñas Porto, Noelia Tarazona, Javier Hernandez Losa, Rodrigo Almeida Toledo

¹The Liquid Biopsy and Biomarker Working Module,the Biomedical Research Network in Cancer (CIBERONC)

Capitoli

00:05Introduction
01:30Serum Preparation for Liquid Biopsy Studies
02:25Plasma Preparation for Liquid Biopsy Studies
04:08Results: Electrophoresis-Based Analysis of cfDNA Isolated from Plasma
04:28Conclusion

Summary

Traduzione automatica

液体活检彻底改变了我们的肿瘤学转化研究方法，样本收集、质量和储存是其成功临床应用的关键步骤。在这里，我们描述了一种标准化且经过验证的下游无循环DNA应用的方案，该协议可应用于大多数转化研究实验室。

Tags

Keywords: Liquid Biopsy Circulating Free DNA Preanalytical Protocol Blood Sample Processing Plasma Extraction Serum Extraction Sample Centrifugation Sample Storage Hemolysis Detection Standardized Procedure Translational Research Clinical Applications Oncology Research

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