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1Laboratory of Genetics,The Salk Institute for Biological Studies, 2Jack H. Skirball Center for Chemical Biology and Proteomics,The Salk Institute for Biological Studies, 3Department of Chemistry and Biochemistry,University of California San Diego, 4Graduate School of Biological Sciences, Section of Molecular Biology,University of California San Diego, 5Department of Integrative Structural and Computational Biology,The Scripps Research Institute
Capitoli
- 00:05Introduction
- 00:28Setting Up the Tilted Data Collection
- 03:23Results: Images of the Grid at Square Magnification with Different Tilt Angles and Hole Exposures and 2D Class Averages Collected at Different Tilt Angles
- 04:27Conclusion
Denne protokol beskriver et generelt og let at implementere skema til vippet enkeltpartikeldataindsamling i kryo-EM-eksperimenter. En sådan procedure er især nyttig til opnåelse af et EM-kort af høj kvalitet for prøver, der lider af præferenceorienteringsbias på grund af overholdelse af luft-vand-grænsefladen.
