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Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures
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Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures

Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures

DOI:
10.3791/64146-v

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08:02 min

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July 27, 2022

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, , ,
1Biology Department,Indian Institute of Science Education and Research, 2Department of Biology, Center for Genomics and Systems Biology,New York University, 3Enveda Therapeutics India Pvt Ltd.

Capitoli

  • 00:04Introduction
  • 00:59Seeding MCF10A Cells in lrECM, PAF Treatment, and Re-Feeding with Fresh Media
  • 03:00Immunofluorescence Study to Detect Phenotypic Changes Induced by Prolonged PAF Exposure
  • 06:05Results: Nuclei of Cells in MCF10A Acini Stained with a Nuclear Stain
  • 07:25Conclusion

Summary

Traduzione automatica

Traduzione automatica

The present protocol describes the setting up of 3D 'on top' cultures of a non-transformed breast epithelial cell line, MCF10A, that has been modified to study Platelet Activating Factor (PAF) induced transformation. Immuno-fluorescence has been used to assess the transformation and is discussed in detail.

Tags

3D Cell CultureLrECMPhospholipid MediatorPAFBreast CancerBiomarkersDrug TargetsTumorigenesisMorphogenesis

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