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Blid isolering af kerner fra hjernevævet hos voksne afrikanske turkise killifisk, en naturligt kortvarig model for aldringsforskning
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1Leonard Davis School of Gerontology,University of Southern California, 2Molecular and Computational Biology Department,USC Dornsife College of Letters, Arts and Sciences, 3Biochemistry and Molecular Medicine Department,USC Keck School of Medicine, 4Epigenetics and Gene Regulation,USC Norris Comprehensive Cancer Center, 5USC Stem Cell Initiative
Capitoli
- 00:05Introduction
- 01:07Nuclei Isolation
- 04:46Quantify Singlet Nuclei, Debris, and Multiplet Proportion by Flow Cytometry
- 07:35Results: Nuclei Quantification and Purity Assessment by Flow Cytometry
- 08:37Conclusion
Her præsenterer vi en protokol til at isolere kerner fra hjernen i den kortlivede hvirveldyrmodel Nothobranchius furzeri til downstream-applikationer såsom enkeltkerne-RNA-sekventering eller enkeltkerneassay til transposase-tilgængeligt kromatin med sekventering (ATAC-seq).
