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Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
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1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital,Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children, 2019RU016,Chinese Academy of Medical Sciences
Capitoli
- 00:05Introduction
- 00:36Isolation of CD14+ Monocytes from PBMCs Using CD14 Microbeads
- 02:38Fluorescently Labeled Antibody Staining
- 03:52Flow Cytometry Parameter Setting
- 04:32Gating
- 06:18Cell Sorting and Collection via Flow Cytometry
- 07:00Results: Comparison of Immune Cell Subpopulations from Patients with Infectious Mononucleosis, Healthy Epstein Barr Virus Carriers, and Uninfected Samples
- 08:14Conclusion
We describe a method combining immunomagnetic beads and fluorescence-activated cell sorting to isolate and analyze defined immune cell subpopulations of peripheral blood mononuclear cells (monocytes, CD4+ T cells, CD8+ T cells, B cells, and natural killer cells). Using this method, magnetic and fluorescently labeled cells can be purified and analyzed.
