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Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model

Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model

DOI:
10.3791/64215-v

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09:48 min

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July 27, 2022

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, , , , , ,
1Division of Pediatric Surgery,Oklahoma Children’s Hospital, 2Oklahoma University Health Sciences Center, 3Department of Surgery,University of Oklahoma Health Sciences Center

Capitoli

  • 00:04Introduction
  • 00:59Generation of Apical‐Out (AO) Enteroids
  • 03:08Induction of Experimental Necrotizing Enterocolitis (NEC)
  • 05:06Measurement of Paracellular Permeability Utilizing Lucifer Yellow (LY)
  • 07:49Results: Determining the Intestinal Permeability Using Lucifer Yellow in an Apical‐Out Enteroid Model
  • 09:07Conclusion

Summary

Traduzione automatica

Traduzione automatica

The present protocol outlines a method that utilizes lucifer yellow in an apical-out enteroid model to determine intestinal permeability. This method can be used to determine paracellular permeability in enteroids that model inflammatory bowel diseases such as necrotizing enterocolitis.

Tags

Lucifer YellowApical-out Enteroid ModelIntestinal PermeabilityParacellular PathwaysNecrotizing EnterocolitisCell Recovery SolutionCentrifugation3D Model24-well PlateAO Enteroids

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