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Cancer Research

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JoVE Journal Cancer Research
Modeling Brain Metastasis by Internal Carotid Artery Injection of Cancer Cells
 

Modeling Brain Metastasis by Internal Carotid Artery Injection of Cancer Cells

Article DOI: 10.3791/64216-v • 10:01 min • August 2nd, 2022
August 2nd, 2022 •
1, 2, 1, 3, 2, *1,4, *1,5
1UQ Centre for Clinical Research, The University of Queensland, 2Centre for Advanced Imaging, Australian Institute for Bioengineering and Nanotechnology and ARC Training Centre for Innovation in Biomedical Imaging Technology, The University of Queensland, 3School of Biomedical Sciences, The University of Queensland, 4Mater Research and Mater Research Institute, The University of Queensland, 5Pathology Queensland, Royal Brisbane Women's Hospital
* These authors contributed equally

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Brain metastasis is a cause of severe morbidity and mortality in cancer patients. Most brain metastasis mouse models are complicated by systemic metastases confounding analysis of mortality and therapeutic intervention outcomes. Presented here is a protocol for internal carotid injection of cancer cells that produces consistent intracranial tumors with minimal systemic tumors.

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Keywords: Brain Metastasis Internal Carotid Artery Injection Cancer Cells Mouse Model BT-474 Cells Cell Culture Cell Preparation Cell Injection
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