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Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium Oxide
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Biologia
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JoVE Journal Biologia
Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium Oxide

Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium Oxide

DOI:
10.3791/64219-v

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07:34 min

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May 12, 2023

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, , ,
1UCD Conway Institute and UCD Institute of Food and Health, School of Agriculture and Food Science,University College Dublin, 2Division of Endocrinology,Cincinnati Children’s Hospital Medical Center, Cincinnati, 3Division of Developmental Biology,Cincinnati Children’s Hospital Medical Center, Cincinnati, 4Department of Pediatrics,University of Cincinnati College of Medicine

Capitoli

  • 00:04Introduction
  • 00:48Lipid Extraction from Adipose Tissue
  • 02:31Preparation of Fatty Acid Methyl Esters (FAMEs) and GCMS Analysis
  • 04:08Deuterium Acetone Exchange of Plasma Samples to Determine Body Water Enrichment
  • 05:04Results: Plasma Body Water Enrichment from D2O, and Brown Adipose Tissue Total Fatty Acid Enrichment and Synthesis after 3 Days of D2O Administration
  • 06:34Conclusion

Summary

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Here, we present an inexpensive quantitative method utilizing deuterium oxide and gas chromatography mass spectrometry (GCMS) for the analysis of total fatty acid de novo lipogenesis in brown adipose tissue in vivo.

Tags

Fatty Acid SynthesisDeuterium OxideNon-invasiveIsotope-labeled GlucoseMetabolic StatusChloroform-methanol ExtractionFatty Acid MethylationGC-MS Analysis

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