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In vivo Imágenes de calcio de campo amplio y dos fotones de un ratón usando una ventana craneal grande
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1Department of Neurophysiology, Faculty of Medicine,University of Yamanashi, 2Department of Neuropharmacology, Faculty of Medicine,University of Yamanashi, 3Yamanashi GLIA center, Interdisciplinary Graduate School of Medicine,University of Yamanashi, 4Department of Neurochemistry, Graduate School of Medicine,The University of Tokyo
Capitoli
- 00:04Introduction
- 00:51Cranial Window Construction
- 03:32Making AAV Film on Plastic Wrap Using Fibroin Solution
- 04:17Results: Evaluation of Cranial Window Made Using the PVDC Wrap Methods
- 05:56Conclusion
El presente protocolo describe la fabricación de una ventana craneal grande (6 x 3 mm2) utilizando envoltura de alimentos, silicona transparente y vidrio de cubierta. Esta ventana craneal permite experimentos in vivo de imágenes de calcio de campo amplio y dos fotones en el mismo ratón.
