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In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents
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JoVE Journal Medicina
In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents

In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents

DOI:
10.3791/64227-v

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09:17 min

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September 07, 2022

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, ,
1Department of Pharmacology and Chemical Biology,University of Pittsburgh School of Medicine

Capitoli

  • 00:05Introduction
  • 00:35Anesthesia and Ganglionic Block
  • 01:29Surgical Procedure: Suprapubic Bladder Catheterization
  • 02:29Transurethral Catheterization
  • 03:43Experimental Setup
  • 05:08Collection of Samples
  • 06:20Quantifying ATP from Collected Samples
  • 07:03Results: Measurement of Urothelial ATP Release In Vivo from the Lumen of the Bladder
  • 08:39Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes the procedure for measuring ATP concentrations in the lumen of the bladder in an anesthetized rodent.

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