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Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique
JoVE Journal
Biologia
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JoVE Journal Biologia
Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique
DOI:

06:14 min

October 06, 2022

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Capitoli

  • 00:04Introduction
  • 00:46Compound Extraction and Separation of the Crude Extract by Normal Phase Adsorption Column
  • 03:04Preparation and Loading of Thin-Layer Chromatogram Plates and Analysis by the Blue-LED Illuminator
  • 04:28Results: Estimating the Yield of Compounds Using the Blue-LED Illumination Technique
  • 05:49Conclusion

Summary

Traduzione automatica

The present protocol developed a method to estimate the yield of compounds on the TLC plate using the blue-LED illumination technique. The advantages of this approach are that it is safe, effective, inexpensive, and allows the researcher to measure multiple samples simultaneously.

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