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Organotypic Cultures of Adult Human Cortex as an Ex vivo Model for Human Stem Cell Transplantation and Validation
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Capitoli
- 00:05Introduction
- 01:14Tissue Collection, Maintenance, Cutting, and Plating
- 03:25Transplantation of the GFP-lt-NES Cells into Organotypic Human Adult Cortical (hACtx) Slices
- 04:57Results: Characterization and Electrophysiological Properties of hACtx Neurons
- 06:49Conclusion
This protocol describes long-term organotypic cultures of adult human cortex combined with ex vivo intracortical transplantation of induced pluripotent stem cell-derived cortical progenitors, which present a novel methodology to further test stem cell-based therapies for human neurodegenerative disorders.
