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小鼠骨髓造血干细胞和祖细胞以及基质生态位细胞的流式细胞术分析
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Biologia
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JoVE Journal Biologia
Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells
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小鼠骨髓造血干细胞和祖细胞以及基质生态位细胞的流式细胞术分析

DOI:
10.3791/64248-v

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08:34 min

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September 28, 2022

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, , , ,
1Instituto de Investigação e Inovação em Saúde (i3S),Universidade do Porto, 2Department of Onco-Hematology,Instituto Português de Oncologia (IPO)-Porto, 3Unit of Biochemistry, Department of Biomedicine,Faculdade de Medicina da Universidade do Porto, 4Porto Comprehensive Cancer Center (P.CCC)

Capitoli

  • 00:04Introduction
  • 00:43Sample Extraction
  • 01:47Sample Processing for Analysis of Hematopoietic Cell Populations in Total Bone Marrow
  • 03:19Sample Processing for the Analysis of Stromal Cell Populations in Total Bone Marrow
  • 05:13Sample Processing for the Analysis of Hematopoietic Cell Populations in Crushed and Flushed Bone Marrow
  • 06:28Results: Phenotypic Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells by Flow Cytometry
  • 07:40Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们描述了一种简单的方案，用于将小鼠骨髓细胞分离和染色为表型造血干细胞和祖细胞以及支持生态位内皮和间充质干细胞。还包括富集位于内膜和中央骨髓区域的细胞的方法。

Tags

Flow CytometryMurineBone MarrowHematopoietic Stem CellsHematopoietic Progenitor CellsStromal CellsNicheEndostealCentral Bone MarrowRBC LysisCell Staining

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