Rivista
/
Biologia
/
Analyse av sultindusert autofagi i Drosophila melanogaster larvefettlegeme
JoVE Journal
Biologia
Author Produced
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Analyzing Starvation-Induced Autophagy in the Drosophila melanogaster Larval Fat Body

Analyse av sultindusert autofagi i Drosophila melanogaster larvefettlegeme

DOI:
10.3791/64282-v

•

06:02 min

•

August 04, 2022

•

,
1Ministry of Education Key Laboratory of Biosystems Homeostasis and Protection and Innovation Center for Cell Signaling Network, Life Sciences Institute,Zhejiang University

Capitoli

  • 00:04Introduction
  • 00:40Drosophila Crossing and Egg Laying
  • 01:54Amino Acid Starvation Induces Autophagy
  • 02:51Third Instar Larvae Dissection and Sample Tissue Processing
  • 04:29Results: The Patterns of GFP‐Atg8a Puncta in Control, Mu1, and Mu2 Clones
  • 05:22Conclusion

Summary

Traduzione automatica

Traduzione automatica

Denne protokollen beskriver induksjon av autofagi i Drosophila melanogaster larvefettlegeme via næringsutarming og analyserer endringer i autofagi ved bruk av transgene fluestammer.

Tags

AutophagyDrosophila MelanogasterLarval Fat BodyClonal AnalysisAmino Acid DepletionThird Instar Larvae20% Sucrose SolutionDissection

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/64282?article_type=v

Dissection of Larval CNS in Drosophila Melanogaster
check_url/it/64282?article_type=v

Drosophila Larval NMJ Dissection
check_url/it/64282?article_type=v

Drosophila Larval NMJ Immunohistochemistry
check_url/it/64282?article_type=v

Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo
check_url/it/64282?article_type=v

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies
check_url/it/64282?article_type=v

Isolation of Drosophila melanogaster Testes
check_url/it/64282?article_type=v

Måling av levetid i<em> Drosophila melanogaster</em
check_url/it/64282?article_type=v

Imaging Gjennom pupal Case of<em> Drosophila melanogaster</em
check_url/it/64282?article_type=v

Måling av Larve Aktiviteten i<em> Drosophila</em> Activity Monitor
check_url/it/64282?article_type=v

Fett kroppen Organ kultur-systemet i Aedes Aegypti, en vektor av Zika Virus

Read Article