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用于评估人iPSC来源心肌细胞结构和收缩变化的杂交细胞分析系统，用于临床前心脏风险评估

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Hybrid Cell Analysis System to Assess Structural and Contractile Changes of Human iPSC-Derived Cardiomyocytes for Preclinical Cardiac Risk Evaluation

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用于评估人iPSC来源心肌细胞结构和收缩变化的杂交细胞分析系统，用于临床前心脏风险评估

DOI:

10.3791/64283-v

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08:03 min

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October 20, 2022

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Bettina Lickiss, Matthias Gossmann, Peter Linder, Ulrich Thomas, Elena Dragicevic, Marta Lemme, Michael George, Niels Fertig, Sonja Stölzle-Feix

¹innoVitro GmbH, ²Nanion Technologies GmbH

Capitoli

00:04Introduction
00:53Plate Coating
01:58Seeding of Human iPSC‐Derived Cardiomyocytes into Flexible 96‐Well Plates (Day 0)
02:56Medium Exchange of Flexible 96‐Well Plates (Day 1)
03:53Final Medium Exchange Before Compound Addition (Day 5–7)
04:21Compound Addition and Data Recording (Day 5–7)
05:47Results: Contractility Assessment, Base Impedance, and EFP Recordings of Human iPSC‐Derived Cardiomyocytes After Drug Treatment
07:35Conclusion

Summary

Traduzione automatica

分析人iPSC来源的心肌细胞的收缩功能和细胞完整性的变化对于非临床药物开发具有重要意义。混合 96 孔细胞分析系统以实时和生理方式处理这两个参数，以获得可靠的、与人类相关的结果，这是安全过渡到临床阶段所必需的。

Tags

Keywords: Hybrid Cell Analysis System IPSC-derived Cardiomyocytes Preclinical Cardiac Risk Evaluation Electrophysiological Assessment Structural Analysis Contractile Properties Drug Discovery High-throughput Testing Cell Seeding EHS Gel Coating Cell Culture Cardiomyocyte Maintenance Medium

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