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太平洋水母干细胞样细胞的荧光原位杂交和5-乙炔基-2'-脱氧尿苷标记
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Fluorescent In Situ Hybridization and 5-Ethynyl-2′-Deoxyuridine Labeling for Stem-Like Cells in the Hydrozoan Jellyfish Cladonema pacificum
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太平洋水母干细胞样细胞的荧光原位杂交和5-乙炔基-2'-脱氧尿苷标记

DOI:
10.3791/64285-v

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08:44 min

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August 03, 2022

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, , ,
1Graduate School of Life Sciences,Tohoku University, 2Graduate School of Pharmaceutical Sciences,The University of Tokyo

Capitoli

  • 00:04Introduction
  • 01:36EdU Incorportaion and Fixation
  • 02:25Fluorescent In Situ Hybridization
  • 06:27Results: EdU and Nanos1 Expression Patterns on the Proximal, Adaxial Side of Cladonema Medusa Tentacle
  • 08:02Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们描述了一种用于可视化水母 Cladonema中干细胞样增殖细胞的协议。与干细胞标记物的全安装荧光 原位 杂交可以检测干细胞样细胞，5-乙炔基-2'-脱氧尿苷标记可以鉴定增殖细胞。一起，可以检测到活跃增殖的干细胞样细胞。

Tags

Fluorescent In Situ Hybridization5-ethynyl-2′-deoxyuridine LabelingStem-like CellsHydrozoan JellyfishCladonema PacificumNanos1 ExpressionEdU LabelingStem Cell HeterogeneityBiologia dello sviluppoRegenerationAdult Stem CellsTentacle BranchingTentacle RegenerationLaboratory Model Organism

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