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改进小鼠噪声性听力损失的实验条件及听力功能和外毛细胞损伤的评估

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage

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改进小鼠噪声性听力损失的实验条件及听力功能和外毛细胞损伤的评估

DOI:

10.3791/64286-v

•

07:13 min

•

February 10, 2023

•

Dian-Shiue Lee, Yu-Ling Pan, Yi-Chao Hsu²

¹Department of Audiology and Speech-Language Pathology,MacKay Medical College, ²Institute of Biomedical Sciences,MacKay Medical College

Capitoli

00:04Introduction
01:04Induction of Noise‐Induced Hearing Loss (NIHL) in Mice
03:07Auditory Brainstem Response (ABR)‐Based Assessment of Hearing Threshold
04:19Microscope Examination
05:18Results: Assessing Hearing Function and Outer Hair Cell Damage in NIHL Mice
06:32Conclusion

Summary

Traduzione automatica

在这里，我们提出了噪声诱导听力损失（NIHL）小鼠模型的方案。为了诱导NIHL，我们开发了一种使用波纹塑料，捕鼠笼和扬声器的新型简单设备。采用听觉脑干反应和免疫荧光成像分别评估听力功能和外毛细胞损伤。

Tags

Noise-induced Hearing Loss Mice ABR Outer Hair Cell Damage Experimental Conditions Hearing Function Assessment Animal Model Sound Exposure Hearing Threshold

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