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Kombination af clearing- og fluorescensmikroskopi til visualisering af ændringer i genekspression og fysiologiske reaktioner på Plasmodiophora brassicae
JoVE Journal
Biologia
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JoVE Journal Biologia
Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae

Kombination af clearing- og fluorescensmikroskopi til visualisering af ændringer i genekspression og fysiologiske reaktioner på Plasmodiophora brassicae

DOI:
10.3791/64297-v

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06:58 min

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August 05, 2022

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, , , , , ,
1Integrative Plant Biology Team,Institute of Plant Genetics Polish Academy of Sciences, 2ZMBP-Center for Plant Molecular Biology,University of Tübingen, 3Department of General Botany, Institute of Experimental Biology, Faculty of Biology,Adam Mickiewicz University

Capitoli

  • 00:05Introduction
  • 01:36Tissue Preparation and Fixation
  • 02:13Tissue Embedding and Sectioning
  • 03:03Vibratome Sectioning and Specimen Clearing
  • 04:03Staining Procedure and Microscopy
  • 04:49Results: Microscopic Analysis of the Biotrophic Interaction and Gene Expression Regulations Between Plasmodiophora brassicae and Arabidopsis Plants
  • 06:06Conclusion

Summary

Traduzione automatica

Traduzione automatica

Den nuværende protokol beskriver en optimeret metode til histologisk observation af galler induceret af Plasmodiophora brassicae. Vibrerende sektioner af hypocotyler ryddes før fluorescensbilleddannelse for at studere involvering af transkriptionsfaktorer og phytohormoner under sygdomsprogression. Denne protokol overvinder begrænsninger for harpiksindlejring, hvilket muliggør plantavisualisering af fluorescerende proteiner.

Tags

Keywords: Plasmodiophora BrassicaeClubrootGene ExpressionFluorescence MicroscopyClearingGallsPhytohormoneLignificationPlant-pathogen InteractionAgarose EmbeddingVibratome Sectioning

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