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Immunology and Infection

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JoVE Journal Immunology and Infection
Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo
 

Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo

Article DOI: 10.3791/64299-v • 07:33 min • September 28th, 2022
September 28th, 2022 •
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1National Engineering Research Centre of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of Chinese PLA

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Here, we present detailed methods for the preparation and evaluation of the nasal self-assembled nanoemulsion tumor vaccine in vitro and in vivo.

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Nanoemulsion Tumor Vaccine In Vitro In Vivo Characteristics Toxicity Efficacy Evaluation Self-assembled Nanoemulsion Disease Therapy Tumor Tropism Specific Tactics Low Systemic Toxicity T-cell Epitope Peptide Mucosal Vaccines Monophosphorol Lipid A DMSO TWEEN 80 I-OVA Squalene MPLA Solution Water Droplets Transparent Mixture BEAS2B Epithelial Cells Complete Growth Medium
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