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1Division of Clinical Pharmacology, Department of Medicine Vanderbilt University Medical Center and Department of Molecular Physiology and Biophysics,Vanderbilt University, 2Division of Pediatric Nephrology Department of Pediatrics,Vanderbilt University Medical Center, 3Department of Pathology, Microbiology, and Immunology,Vanderbilt University Medical Center
Capitoli
- 00:05Introduction
- 00:29Aseptic Collection of Mouse Fecal Samples
- 01:21Fecal Matter Transplantation (FMT)
- 02:06Systolic Blood Pressure Measurements
- 02:47Assessment of FMT to Cardiovascular Changes and Making Gut Intestine Swiss-Rolls
- 05:46Results: Effects of High and Low-Salt Diet, Fecal Microbiota Transplantation from High-Salt Fed Mice, and Immunohistochemistry on the Ileum
- 07:07Conclusion
Målet her er å skissere en protokoll for å undersøke mekanismene for dysbiose ved kardiovaskulær sykdom. Denne artikkelen diskuterer hvordan man aseptisk samler og transplanterer murine fekale prøver, isolerer tarmene og bruker "Swiss-roll" -metoden, etterfulgt av immunfargingsteknikker for å forhøre endringer i mage-tarmkanalen.
