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脑室微注射脂多糖到斑马鱼幼虫中以评估神经炎症和神经毒性
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Neuroscienze
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JoVE Journal Neuroscienze
Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity
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脑室微注射脂多糖到斑马鱼幼虫中以评估神经炎症和神经毒性

DOI:
10.3791/64313-v

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07:31 min

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August 23, 2022

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,
1State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences,University of Macau, Avenida da Universidade, 2Department of Pharmaceutical Sciences, Faculty of Health Sciences,University of Macau, Avenida da Universidade

Capitoli

  • 00:05Introduction
  • 00:39Preparing and Mounting Zebrafish for Microinjections
  • 02:22Injecting the Brain Ventricle
  • 03:08Imaging
  • 03:43Determination of Gene Expression Markers
  • 04:44Zebrafish Locomotive Behavioral Assay
  • 05:34Results: Lipopolysaccharide-Induced Neuroinflammation to Assess the Levels of Proinflammatory Mediators
  • 07:02Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议展示了在斑马鱼幼虫模型中将脂多糖显微注射到脑室区域，以研究由此产生的神经炎症反应和神经毒性。

Tags

Brain VentricleMicroinjectionLipopolysaccharideNeuroinflammationNeurotoxicityZebrafishIn Vivo ModelAnti-neuroinflammatory DrugsGlass CapillaryMicromanipulatorAgaroseAnesthesia

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