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Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography
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Biochimica
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JoVE Journal Biochimica
Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography

Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography

DOI:
10.3791/64322-v

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06:26 min

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January 06, 2023

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, , ,
1Peak Proteins Ltd.

Capitoli

  • 00:04Introduction
  • 00:37Sample Preparation for Fluorescent Size Exclusion Chromatography (FSEC)
  • 02:06Size Exclusion Chromatography (SEC)
  • 03:33Fluorescent Trace Collection and Analysis
  • 04:17Results: Fluorescent Size Exclusion Chromatography (FSEC) on Membrane Proteins for Downstream Functional and Structural Analysis Quality Assessment
  • 05:48Conclusion

Summary

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The present protocol describes a procedure to perform fluorescent size exclusion chromatography (FSEC) on membrane proteins to assess their quality for downstream functional and structural analysis. Representative FSEC results collected for several G-protein coupled receptors (GPCRs) under detergent-solubilized and detergent-free conditions are presented.

Tags

Membrane ProteinSEC ProfileFluorescenceFSECPurificationSolubilizationDDMCHSCentrifugationFPLCSize-exclusion Chromatography

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