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硬骨模型日本青鳉鱼（Oryzias latipes）中的精子收集和计算机辅助精子分析

JoVE Journal
Biologia
Author Produced

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JoVE Journal Biologia

Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka (Oryzias latipes)

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硬骨模型日本青鳉鱼（Oryzias latipes）中的精子收集和计算机辅助精子分析

DOI:

10.3791/64326-v

•

06:49 min

•

October 06, 2022

•

Lauren Closs, Amin Sayyari, Romain Fontaine

¹Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine,Norwegian University of Life Sciences, ²Department of Production Animal Clinical Sciences, Faculty of Veterinary Medicine,Norwegian University of Life Sciences

Capitoli

00:04Introduction
00:50Sperm Collection by Abdominal Massage
02:14Sperm Collection by Testes Dissection
03:02Computer-Assisted Sperm Analysis (CASA)
04:33Results: Determination of Sperm Motility Under Various Factors
06:11Conclusion

Summary

Traduzione automatica

本文介绍了两种从小型模式鱼青鳉鱼（Oryzias latipes）收集精子的快速有效方法，以及使用计算机辅助精子分析（CASA）可靠地评估精子质量的方案。

Tags

Keywords: Sperm Collection Computer-assisted Sperm Analysis Japanese Medaka Oryzias Latipes Abdominal Massage Sperm Quality Fertilization Success Milt Cryo Preservation Anesthesia Dissecting Microscope Activating Solution Testes

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