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Sædopsamling og computerassisteret sædanalyse i Teleost-modellen japansk Medaka (Oryzias latipes)
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Biologia
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JoVE Journal Biologia
Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka (Oryzias latipes)

Sædopsamling og computerassisteret sædanalyse i Teleost-modellen japansk Medaka (Oryzias latipes)

DOI:
10.3791/64326-v

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06:49 min

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October 06, 2022

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, ,
1Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine,Norwegian University of Life Sciences, 2Department of Production Animal Clinical Sciences, Faculty of Veterinary Medicine,Norwegian University of Life Sciences

Capitoli

  • 00:04Introduction
  • 00:50Sperm Collection by Abdominal Massage
  • 02:14Sperm Collection by Testes Dissection
  • 03:02Computer-Assisted Sperm Analysis (CASA)
  • 04:33Results: Determination of Sperm Motility Under Various Factors
  • 06:11Conclusion

Summary

Traduzione automatica

Traduzione automatica

Denne artikel beskriver to hurtige og effektive metoder til opsamling af sæd fra den lille model fisk medaka (Oryzias latipes), samt en protokol til pålidelig vurdering af sædkvalitet ved hjælp af computerassisteret sædanalyse (CASA).

Tags

Sperm CollectionComputer-assisted Sperm AnalysisJapanese MedakaOryzias LatipesAbdominal MassageSperm QualityFertilization SuccessMiltCryo PreservationAnesthesiaDissecting MicroscopeActivating SolutionTestes

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