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Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka (Oryzias latipes)
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Biologia
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JoVE Journal Biologia
Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka (Oryzias latipes)

Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka (Oryzias latipes)

DOI:
10.3791/64326-v

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06:49 min

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October 06, 2022

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, ,
1Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine,Norwegian University of Life Sciences, 2Department of Production Animal Clinical Sciences, Faculty of Veterinary Medicine,Norwegian University of Life Sciences

Capitoli

  • 00:04Introduction
  • 00:50Sperm Collection by Abdominal Massage
  • 02:14Sperm Collection by Testes Dissection
  • 03:02Computer-Assisted Sperm Analysis (CASA)
  • 04:33Results: Determination of Sperm Motility Under Various Factors
  • 06:11Conclusion

Summary

Traduzione automatica

Traduzione automatica

This article describes two quick and efficient methods for collecting sperm from the small model fish medaka (Oryzias latipes), as well as a protocol for reliably assessing sperm quality using computer-assisted sperm analysis (CASA).

Tags

Keywords: Sperm CollectionComputer-assisted Sperm AnalysisJapanese MedakaOryzias LatipesAbdominal MassageSperm QualityFertilization SuccessMiltCryo PreservationAnesthesiaDissecting MicroscopeActivating SolutionTestes

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