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The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion
JoVE Journal
Biologia
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JoVE Journal Biologia
The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion

The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion

DOI:
10.3791/64338-v

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06:14 min

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November 30, 2022

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, ,
1Division of Endocrinology and Metabolism, Department of Medicine, School of Medicine,University of Pittsburgh

Capitoli

  • 00:05Introduction
  • 01:22Surgery and Experimental Design
  • 04:04Collection of Luminal Contents and Mucosal Tissue
  • 04:46Results: Analyzing the Mesenteric Lymph Triglyceride Concentration and Flow Rate
  • 05:13Conclusion

Summary

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The present protocol describes a detailed surgical protocol for isolating flowing intestinal lymph in response to intraduodenal nutrient infusions in mice. This allows for the physiological determination of total intestinal lipid absorption and chylomicron synthesis and secretion in response to various experimental nutrients.

Tags

Mesenteric LymphDietary Lipid AbsorptionChylomicron SecretionLymph KineticsDietary Lipid MetabolismMouse SurgeryLymph CannulationDuodenal InfusionIn Vivo Study

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