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分离小鼠肠系膜流动淋巴液以量化膳食脂质吸收和乳糜微粒分泌的体内动力学

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion

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分离小鼠肠系膜流动淋巴液以量化膳食脂质吸收和乳糜微粒分泌的体内动力学

DOI:

10.3791/64338-v

•

06:14 min

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November 30, 2022

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Nikolaos L. Dedousis, Lihong Teng, Alison B. Kohan

¹Division of Endocrinology and Metabolism, Department of Medicine, School of Medicine,University of Pittsburgh

Capitoli

00:05Introduction
01:22Surgery and Experimental Design
04:04Collection of Luminal Contents and Mucosal Tissue
04:46Results: Analyzing the Mesenteric Lymph Triglyceride Concentration and Flow Rate
05:13Conclusion

Summary

Traduzione automatica

本协议描述了一种详细的手术方案，用于分离流动的肠淋巴以响应小鼠十二指肠内营养输注。这允许对响应各种实验营养素的总肠道脂质吸收和乳糜微粒合成和分泌进行生理测定。

Tags

Keywords: Mesenteric Lymph Dietary Lipid Absorption Chylomicron Secretion Lymph Kinetics Dietary Lipid Metabolism Mouse Surgery Lymph Cannulation Duodenal Infusion In Vivo Study

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