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激光诱导动作电位样测量微电极阵列上的心肌细胞，以提高安全药理学的预测性

JoVE Journal
Medicina

This content is Free Access.

JoVE Journal Medicina

Laser-Induced Action Potential-Like Measurements of Cardiomyocytes on Microelectrode Arrays for Increased Predictivity of Safety Pharmacology

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激光诱导动作电位样测量微电极阵列上的心肌细胞，以提高安全药理学的预测性

DOI:

10.3791/64355-v

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10:41 min

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September 13, 2022

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Capitoli

00:04Introduction
00:49Induced Pluripotent Stem Cell-Derived Cardiomyocytes Preparation
02:24MEA Recordings
04:22Laser-Induced Cell Poration
06:28Drug Handling and Application
08:01Results: Pharmacological Modulation of the Cardiac Action Potential
10:03Conclusion

Summary

Traduzione automatica

激光穿孔和微电极阵列（MEA）的组合允许对培养的原代和干细胞来源的心肌细胞进行类似动作电位的记录。波形形状提供了比标准记录更深入的测试化合物作用方式的洞察力。它将膜片钳和MEA读数连接在一起，以进一步优化未来的心脏安全研究。

Tags

Keywords: Laser-induced Action Potential Microelectrode Arrays Cardiomyocytes Intracellular Action Potentials Field Potential Recordings Stem Cell Technology Cardiac Disease Arrhythmias Cell Cultivation Fibronectin Plating Medium MEA Setup Laser Focus Noise Reduction

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