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Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
JoVE Journal
Biologia
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JoVE Journal Biologia
Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

DOI:
10.3791/64363-v

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09:14 min

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September 13, 2022

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, , , ,
1Cell Biology and Biophysics,European Molecular Biology Laboratory, 2Collaboration for joint PhD degree between EMBL and Heidelberg University,Faculty of Biosciences, 3EMBL Imaging Centre,European Molecular Biology Laboratory

Capitoli

  • 00:04Introduction
  • 01:04Dissection and Fixation
  • 02:52Incubation with Antibodies
  • 04:00Mounting Samples on a Coverslip
  • 05:27Imaging
  • 07:30Results: Visualization of Synaptonemal Complex Using Super‐Resolution Microscopy
  • 08:34Conclusion

Summary

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Super-resolution microscopy can provide a detailed insight into the organization of components within the synaptonemal complex in meiosis. Here, we demonstrate a protocol to resolve individual proteins of the Caenorhabditis elegans synaptonemal complex.

Tags

Super-resolution MicroscopySynaptonemal ComplexCaenorhabditis ElegansGermlineMeiosisImmunostainingSingle Molecule Localization MicroscopyDissectionFixationAntibody Staining

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