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Tracking Bispecific Antibody-Induced T Cell Trafficking Using Luciferase-Transduced Human T Cells
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JoVE Journal Ricerca sul cancro
Tracking Bispecific Antibody-Induced T Cell Trafficking Using Luciferase-Transduced Human T Cells

Tracking Bispecific Antibody-Induced T Cell Trafficking Using Luciferase-Transduced Human T Cells

DOI:
10.3791/64390-v

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10:19 min

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May 12, 2023

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, ,
1Department of Pediatrics,Memorial Sloan Kettering Cancer Center

Capitoli

  • 00:04Introduction
  • 00:39Transfection of 293T Cells with Luciferase and Harvest of Viral Supernatant
  • 03:22Expansion and Transduction of Activated Human T Cells with Luciferase
  • 06:20Engraftment of Luciferase‐Transduced T cells in Immunocompromised Mice and In Vivo Imaging
  • 07:41Results: Evaluating the Anti‐Tumor Efficacy and Mechanism of T Cell‐Engaging Bispecific Antibodies
  • 09:51Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we describe a method for transducing human T cells with luciferase to facilitate in vivo tracking of bispecific antibody-induced T cell trafficking to tumors in studies to evaluate the anti-tumor efficacy and mechanism of T cell-engaging bispecific antibodies.

Tags

Bispecific AntibodyT Cell TraffickingLuciferase-transducedIn Vivo TrackingT Cell PersistenceImmune Cell Tracking293T Cell CultureViral TransductionT Cell Activation

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