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1Mechanobiology Institute (MBI),National University of Singapore, 2Biomedical Engineering Department,National University of Singapore, 3Department of Biological Sciences,National University of Singapore, 4IRL 3639 CNRS, 5Department of Microbiology & Immunology, Immunology Translational Research Program, Yong Loo Lin School of Medicine,National University of Singapore
Capitoli
- 00:04Introduction
- 00:49PDMS Mold Dicing and Production of the UV‐Curable, Adhesive, Textured Layer
- 02:19Coverslip Substrate Preparation
- 03:34Transferring the Textured Film to the Final Substrate
- 04:33Cell Seeding, Release, and Collection of the Organoids
- 06:29Results: Fabrication of the Microwell Culture Dish for a High‐Density Organoid Culture and Differentiation
- 07:00Conclusion
Dette papir præsenterer en fabrikationsprotokol for en ny type kultursubstrat med hundredvis af mikrobeholdere pr. mm2, hvor organoider kan dyrkes og observeres ved hjælp af mikroskopi med høj opløsning. Protokollerne for cellesåning og immunfarvning er også detaljerede.
