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使用碎裂、均质化和连续过滤的组合从牛卵巢中分离小的前窦卵泡
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Biologia
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JoVE Journal Biologia
Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration
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使用碎裂、均质化和连续过滤的组合从牛卵巢中分离小的前窦卵泡

DOI:
10.3791/64423-v

•

09:11 min

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September 27, 2022

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, , ,
1Department of Animal Science,University of California Davis

Capitoli

  • 00:05Introduction
  • 00:55Ovary Preparation and Chopping Procedure
  • 03:52Homogenization Procedure
  • 04:56Filtration Procedure, Searching, and Collecting Follicles
  • 07:08Results: Follicle Isolation from Bovine Ovaries and Immunofluorescent Localization of Proteins
  • 08:28Conclusion

Summary

Traduzione automatica

Traduzione automatica

推进前窦卵泡生成的研究需要从单个卵巢中分离卵泡的有效方法。这里介绍的是一种简化的机械方案，用于使用组织切碎器和均质器从牛卵巢中分离卵泡。该方法允许从单个卵巢中收集大量活的前窦卵泡。

Tags

Keywords: Bovine OvaryPreantral FolliclesFolliculogenesisFertility PreservationOvarian BiopsyFragmentationHomogenizationFiltrationIsolation ProtocolCortexMedullaScalpelPBS

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